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Marios Andreou, Costas Kadis, Pinelopi Delipetrou, Kyriacos Georghiou (2015) Conservation biology of Chionodoxa lochiae and Scilla morrisii (Asparagaceae): Two priority bulbous plant species of the European Union in Cyprus. Global Ecology and Conservation 3: 511–525
Abstract: This paper presents data regarding conservation biology of Chionodoxa lochiae and Scilla morrisii; two threatened endemic plants of Cyprus, which are included as priority species in Annex II of the Habitats Directive. The population size and geographical distribution of the species were monitored for three years. C. lochiae was recorded in ten locations and S. morrisii was recorded in five locations. C. lochiae occurs in Pinus forests with/without Quercus alnifolia understory or in forest margins and riparian vegetation with Platanus orientalis. Favorable habitat of S. morrisii is the understory of Quercus infectoria stands and the Pistacia terebinthus-Quercus coccifera-Styrax officinalis shrubs. The distribution pattern of the species seems to follow habitat availability. Fecundity and Relative Reproductive Success of C. lochiae were stable and low, while in S. morrisii were constantly high. The lack of pollinators seems to be the main cause of the low sexual reproduction of C. lochiae. The germination strategy for both species is dependent on temperature. Some of the seeds are dormant and dormancy is broken by nitrates. The investigation of certain aspects of the biology of the two species yielded the information needed to identify the critical aspects affecting their survival and to propose sound conservation measures.

Athanasopoulos A, Gournas C, Amillis S, Sophianopoulou V (2015) Characterization of AnNce102 and its role in eisosome stability and sphingolipid biosynthesis. Sci Rep 5:15200.

Abstract: The plasma membrane is implicated in a variety of functions, whose coordination necessitates highly dynamic organization of its constituents into domains of distinct protein and lipid composition. Eisosomes, at least partially, mediate this lateral plasma membrane compartmentalization. In this work, we show that the Nce102 homologue of Aspergillus nidulans colocalizes with eisosomes and plays a crucial role in density/number of PilA/SurG foci in the head of germlings. In addition we demonstrate that AnNce102 and PilA negatively regulate sphingolipid biosynthesis, since their deletions partially suppress the thermosensitivity of basA mutant encoding sphingolipid C4-hydroxylase and the growth defects observed upon treatment with inhibitors of sphingolipid biosynthesis, myriocin and Aureobasidin A. Moreover, we show that YpkA repression mimics genetic or pharmacological depletion of sphingolipids, conditions that induce the production of Reactive Oxygen Species (ROS), and can be partially overcome by deletion of pilA and/or annce102 at high temperatures. Consistent with these findings, pilAΔ and annce102Δ also show differential sensitivity to various oxidative agents, while AnNce102 overexpression can bypass sphingolipid depletion regarding the PilA/SurG foci number and organization, also leading to the mislocalization of PilA to septa.
Christodoulou M., Meletiou-Christou M.S., Parmakelis A., Economou-Amilli A. & A. Pantazidou (2015) Further findings from Daveli Cave (Attica, Greece) enhancing the establishment of the genus Oculatella (Pseudanabaenaceae, Cyanobacteria). Phytotaxa, 202 (3): 169-184.
Abstract: In this study, cyanobacterial assemblages were collected from the limestone cave Daveli (Attica, Greece) during an annual survey (2010–2011). Four sampling areas were selected from the cave entrance inwards, and the main abiotic parameters (PAR, T, RH) were measured at each site. A portion of the collected material was fixed with formaldehyde solution and another part was maintained alive for culturing and microscopic observations. XRD analysis was applied for analyzing the chemical composition of the limestone substrate. Microscopic observation (LM) revealed the presence of a wealth of Cyanobacteria including a brownish-violet colored filamentous morphotype with a large granulated ‘ocellus’ in the apical cell, features resembling the newly (2012) established genus Oculatella of the family Pseudanabaenaceae. This morphotype was isolated in unialgal cultures for a further morphological (SEM and TEM), molecular (16S rRNA) and biochemical analysis (FAME). The 16S rRNA sequence generated from the specimen revealed a 99% similarity with Leptolyngbya sp. VRUC135 (GenBank–X84809), a strain now recognized as the type species (Oculatella subterranea) of Oculatella. Taking a step forward, we proceeded to the determination of fatty acid composition by Gas Chromatography which revealed the dominance of palmitic acid (16:0) in all isolates followed by palmitoleic acid (16:1), linoleic acid (18:2), and oleic acid (18:1), while tridecylic acid (13:0), myristic acid (14:0), isomers of C15, C17, C20 and C22, stearic acid (18:0), linolenic acid (18:3), as well as an unknown fatty acid were found in traces. These new findings corroborate the establishment of the new genus Oculatella and provide information on the fatty acid composition of the species Oculatella subterranea.

E.N. Daskalakou, C.A. Thanos and K. Georghiou (2015) Seed biology, reproductive phenology and conservation of Cedrus brevifolia, a threatened endemic tree of Cyprus. Seed Sci. & Technol., 43, 3, 378-389. http://doi.org/10.15258/sst.2015.43.3.05

Abstract: Cone and seed morphometric characteristics, seed germination behaviour and seed viability during storage were studied in Cedrus brevifolia, a threatened endemic forest tree of Cyprus. The number of seeds per cone, seed mass and number of cotyledons per seed followed normal distributions. Seeds germinated promptly in darkness at 20°C after eight weeks of prechilling at 5°C. Seed germination under conditions simulating those prevailing in the field from December to May in Cyprus (wet period) is manifested in early spring (February-March). Far red light does not inhibit seed germination, indicating seedling emergence in the field irrespective of canopy light conditions. Seed longevity was extended by low moisture content and low temperature. Therefore, the data obtained on seed germination and preservation of seed viability contribute to both the understanding of the germination mechanisms in the field and the proper management (in situ and ex situ) of Cyprian cedar.
Evangelinos M, Anagnostopoulos G, Karvela-Kalogeraki I, Stathopoulou PM, Scazzocchio C, Diallinas G. (2015) Minos as a novel Tc1/mariner-type transposable element for functional genomicanalysis in Aspergillus nidulans. Fungal Genet Biol 2015 81:1-11.
Abstract: Transposons constitute powerful genetic tools for gene inactivation, exon or promoter trapping and genome analyses. The Minos element from Drosophila hydei, a Tc1/mariner-like transposon, has proved as a very efficient tool for heterologous transposition in several metazoa. In filamentous fungi, only a handful of fungal-specific transposable elements have been exploited as genetic tools, with the impala Tc1/mariner element from Fusarium oxysporum being the most successful. Here, we developed a two-component transposition system to manipulate Minos transposition in Aspergillus nidulans (AnMinos). Our system allows direct selection of transposition events based on re-activation of niaD, a gene necessary for growth on nitrate as a nitrogen source. On average, among 10(8) conidiospores, we obtain up to ~0.8×10(2) transposition events leading to the expected revertant phenotype (niaD(+)), while ~16% of excision events lead to AnMinos loss. Characterized excision footprints consisted of the four terminal bases of the transposon flanked by the TA target duplication and led to no major DNA rearrangements. AnMinos transposition depends on the presence of its homologous transposase. Its frequency was not significantly affected by temperature, UV irradiation or the transcription status of the original integration locus (niaD). Importantly, transposition is dependent on nkuA, encoding an enzyme essential for non-homologous end joining of DNA in double-strand break repair. AnMinos proved to be an efficient tool for functional analysis as it seems to transpose in different genomic loci positions in all chromosomes, including a high proportion of integration events within or close to genes. We have used Minos to obtain morphological and toxic analogue resistant mutants. Interestingly, among morphological mutants some seem to be due to Minos-elicited over-expression of specific genes, rather than gene inactivation.
D. Gkikas, A. Argiropoulos, S. Rhizopoulou (2015) Epidermal focusing of light and modelling of reflectance infloral-petals with conically shaped epidermal cells. Flora 212: 38–45
Abstract: A model of floral reflectance of petals with conically-shaped epidermal cells is presented for Neriumoleander and Oxalis pes-caprae. The model was achieved by combined microscopic-scale structures andoptical properties of petals; the model theory was based on concepts of physical laws, analytic geometry,vector analysis and micro-optics. The model is shown to fit experimental data of floral reflectance. Con-ically shaped, adaxial, epidermal cells of petals have focal regions, where incident light rays are focusedon the centre of cells. Within tissues light is selectively channelled into sites containing light absorbingpigments. Particular attention was given to consequences of focusing of light within conical, epidermalcells of petals with respect to blossoming regulated by photoperiod, which acts to insure that floweropening occurs during suitable, environmental conditions.

Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM (2015) Sul1 and Sul2 Sulfate Transceptors Signal to Protein Kinase A upon Exit of Sulfur Starvation. J Biol Chem. 2015 Feb 27. pii: jbc.M114.629022

Abstract: Sulfate is an essential nutrient with pronounced regulatory effects on cellular metabolism and proliferation. Little is known, however, about how sulfate is sensed by cells. Sul1 and Sul2 are sulfate transporters in the yeast Saccharomyces cerevisiae, strongly induced upon sulfur starvation and endocytosed upon addition of sulfate. We reveal Sul1,2-dependent activation of protein kinase A (PKA) targets upon sulfate-induced exit from growth arrest after sulfur starvation. We provide two major arguments in favor of Sul1 and Sul2 acting as transceptors for signaling to PKA. First, the sulfate analogue, D-glucosamine 2-sulfate, acted as a non-transported agonist of signaling by Sul1 and Sul2. Second, mutagenesis to Gln of putative H+-binding residues, Glu427 in Sul1 or Glu443 in Sul2, abolished transport without affecting signaling. Hence, Sul1,2 can function as pure sulfate sensors. Sul1E427Q and Sul2E443Q are also deficient in sulfate-induced endocytosis, which can therefore be uncoupled from signaling. Overall, our data suggest that transceptors can undergo independent conformational changes each responsible for triggering different downstream processes. The Sul1 and Sul2 transceptors are the first identified plasma membrane sensors for extracellular sulfate. High-affinity transporters induced upon starvation for their substrate may generally act as transceptors during exit from starvation.
Stelios Katsanevakis, Ivan Deriu, Fabio D’Amico, Ana Luísa Nunes, Sara Pelaez Sanchez, Fabio Crocetta, Margarita Arianoutsou et al. 2015. European Alien Species Information Network (EASIN): supporting European policies and scientific research. Management of Biological Invasions 6(2): 147–157
Abstract: The European Alien Species Information Network (EASIN) was launched in 2012 by the European Commission to facilitate the exploration of existing alien species information and to assist the implementation of European policies on biological invasions. At the core of EASIN, there is an inventory of all known alien and cryptogenic species in Europe (the EASIN Catalogue, herein published), which includes relevant information, such as taxonomic classification, pathways of introduction, year and country of first introduction. Spatial records of species occurrence in Europe are stored in the EASIN geo-databases, integrating data from many data providers and the literature. All this information is publicly available through a widget framework, providing easy to use and flexible tools for searching and mapping. The EASIN datasets have been used for pan European or regional assessments of pathways and gateways of alien invasions, towards the fulfillment of the related targets of the Convention on Biological Diversity and of European policies. Moreover, in support of the new EU Regulation on the prevention and management of the introduction and spread of invasive alien species in Europe, an Early Warning and Rapid Response System is being developed by EASIN.
Koukos Danae, Meletiou-Christou Maria-Sonia, Rhizopoulou Sophia (2015) Leaf surface wettability and fatty acid composition of Arbutus unedo and Arbutus andrachne grown under ambient conditions in a natural macchia. Acta Botanica Gallica: Botany Letters, DOI: 10.1080/12538078.2015.1039579
Abstract: Features of the adaxial and abaxial surface microstructure of Arbutus unedo L. and Arbutus andrachne L. are evaluated as possible consistent parameters contributing to the wetness of leaves. The abaxial leaf surface of A. andrachne and A. unedo was determined to be more hydrophobic than the adaxial leaf surface. Hydrophobicity may be of particular importance for the ecophysiological status of the hypostomatic leaves of both Arbutus species, which exhibit a long lifespan and are exposed to various environmental stimuli. Water repellence may also be correlated to the increased presence of surface wax. Lipid analysis of the leaves of both plant species revealed an abundance of α-linolenic acid, with palmitic acid as the second major contributor, followed by linoleic and oleic acid. Oleic and linoleic acid were present in slightly larger percentages in A. andrachne compared with A. unedo, whereas myristic, palmitic and α-linolenic acids were found in elevated percentages in A. unedo. The fatty acid composition analysis of the leaf wax of A. andrachne and A. unedo ranged from C16 to C26, with fatty acids of an even longer chain length detected in the case of A. andrachne. Despite the similar fatty acid composition of total lipids, the composition of the wax fraction showed differences between the two Arbutus species, which may partly contribute to the foliar surface properties of the two species.

Krypotou E, Evangelidis T, Bobonis J, Pittis AA, Gabaldón T, Scazzocchio C, Mikros E, Diallinas G. (2015) Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Mol Microbiol. 2015 Feb 24. doi: 10.1111/mmi.12982

Abstract: NCS1 proteins are H+ /Na+ symporters specific for the uptake of purines, pyrimidines and related metabolites. In this article we study the origin, diversification and substrate specificity of fungal NCS1 transporters. We show that the two fungal NCS1 subfamilies, Fur and Fcy, and plant homologues, originate through independent horizontal transfers from prokaryotes, and that expansion by gene duplication led to the functional diversification of fungal NCS1. We characterized all Fur proteins of the model fungus Aspergillus nidulans and discovered novel functions and specificities. Homology modelling, substrate docking, molecular dynamics and systematic mutational analysis in three Fur transporters with distinct specificities identified residues critical for function and specificity, located within a major substrate binding site, in transmembrane segments TMS1, TMS3, TMS6 and TMS8. Most importantly, we predict and confirm that residues determining substrate specificity are located not only in the major substrate binding site, but also in a putative outward-facing selective gate. Our evolutionary and structure-function analysis contributes in the understanding of the molecular mechanisms underlying the functional diversification of eukaryotic NCS1 transporters, and in particular, forward the concept that selective channel-like gates might contribute to substrate specificity.
Lamprinou V., Trifinopoulou K., Velonakis E.N., Vatopoulos A., Antonopoulou S., Fragopoulou E. Pantazidou A. & A. Economou-Amilli (2015) Cave Cyanobacteria showing antibacterial activity. International Journal of Speleology, 44(3): 231-238.
Abstract: Cave Cyanobacteria - thriving in an ‘extreme’ environment with interesting species biodiversity - are supposed to be a potential source of bioactive compounds. Lipid extracts from pure cultures of two recently established Cyanobacteria from Greek caves, Toxopsis calypsus and Phormidium melanochroun, were used for antibacterial screening against human pathogenic bacteria (reference and clinical isolates). Antimicrobial Susceptibility testing for both taxa was carried out using the disc-diffusion (Kirby Bauer) method, while preliminary data applying the standard broth microdilution method for the determination of the Minimal Inhibitory Concentration (MIC) are given only for T. calypsus. Antibacterial activity was demonstrated against the Gram-positive clinical and reference bacteria, mostly pronounced in enterococci; no activity was observed against the Gram-negative bacteria. The above screening is the first record of antibacterial activity from lipid extracts of cave Cyanobacteria enhancing the importance of cave microbiota and the necessity for cave conservation.
Martzoukou O, Karachaliou M, Yalelis V, Leung J, Byrne B, Amillis S, Diallinas G. (2015) Oligomerization of the UapA Purine Transporter Is Critical for ER-Exit, Plasma Membrane Localization and Turnover. J Mol Biol 427(16):2679-96.
Abstract: Central to the process of transmembrane cargo trafficking is the successful folding and exit from the ER (endoplasmic reticulum) through packaging in COPII vesicles. Here, we use the UapA purine transporter of Aspergillus nidulans to investigate the role of cargo oligomerization in membrane trafficking. We show that UapA oligomerizes (at least dimerizes) and that oligomerization persists upon UapA endocytosis and vacuolar sorting. Using a validated bimolecular fluorescence complementation assay, we provide evidence that a UapA oligomerization is associated with ER-exit and turnover, as ER-retained mutants due to either modification of a Tyr-based N-terminal motif or partial misfolding physically associate but do not associate properly. Co-expression of ER-retained mutants with wild-type UapA leads to in trans plasma membrane localization of the former, confirming that oligomerization initiates in the ER. Genetic suppression of an N-terminal mutation in the Tyr motif and mutational analysis suggest that transmembrane α-helix 7 affects the oligomerization interface. Our results reveal that transporter oligomerization is essential for membrane trafficking and turnover and is a common theme in fungi and mammalian cells.
Ioannis Mitsopoulos, Giorgos Mallinis, Anna Karali, Christos Giannakopoulos and Margarita Arianoutsou 2015. Mapping fire behaviour under changing climate in a Mediterranean landscape in Greece. Regional Environmental Change. Environmental Management 55: 900–915 DOI 10.1007/s00267-014-0432-6
Abstract: Understanding how future climate periods influence fire behaviour is important for organizing fire suppression strategy and management. The meteorological factors are the most critical parameters affecting fire behaviour in natural landscapes; hence, predicting climate change effects on fire behaviour could be an option for optimizing firefighting resource management. In this study, we assessed climate change impacts on fire behaviour parameters (rate of fire growth, rate of spread and fireline intensity) for a typical Mediterranean landscape of Greece. We applied the minimum travel time fire simulation algorithm by using the FlamMap software to characterize potential response of fire behaviour for three summer periods. The results consisted of simulated spatially explicit fire behaviour parameters of the present climate (2000) and three future summer periods of 2050, 2070 and 2100, under the A1B emissions scenario. Statistical significant differences in simulation outputs among the four examined periods were obtained by using the Tukey’s significance test. Statistical significant differences were mainly obtained for 2100 compared to the present climate due to the significant projected increase in the wind speed by the end of the century. The analysis and the conclusions of the study can be important inputs for fire suppression strategy and fire management (deployment of fire suppression resources, firefighter safety and exposure, transportation logistics) quantifying the effect that the expected future climate periods can have on fire suppression difficulty in Mediterranean landscapes.

Krypotou E, Scazzocchio C, Diallinas G. (2015) Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genet Biol.2015 Feb;75:56-63.

Abstract: The Nucleobase-Ascorbate Transporter (NAT) family includes members in nearly all domains of life. Functionally characterized NAT transporters from bacteria, fungi, plants and mammals are ion-coupled symporters specific for the uptake of purines, pyrimidines and related analogues. The characterized mammalian NATs are specific for the uptake of L-ascorbic acid. In this work we identify in silico a group of fungal putative transporters, named UapD-like proteins, which represent a novel NAT subfamily. To understand the function and specificity of UapD proteins, we cloned and functionally characterized the two Aspergillus brasiliensis NAT members (named AbUapC and AbUapD) by heterologous expression in Aspergillus nidulans. AbUapC represents canonical NATs (UapC or UapA), while AbUapD represents the new subfamily. AbUapC is a high-affinity, high-capacity, H+/xanthine–uric acid transporter, which can also recognize other purines with very low affinity. No apparent transport function could be detected for AbUapD. GFP-tagging showed that, unlike AbUapC which is localized in the plasma membrane, AbUapD is ER-retained and degraded in the vacuoles, a characteristic of misfolded proteins. Chimeric UapA/AbUapD molecules are also turned-over in the vacuole, suggesting that UapD includes intrinsic peptidic sequences leading to misfolding. The possible evolutionary implication of such conserved, but inactive proteins is discussed.
S. Rhizopoulou and Georgios Kapolas (2015) In situ study of deep roots of Capparis spinosa L. during the dry season: Evidence from a natural “rhizotron” in the ancient catacombs of Milos Island (Greece). Journal of Arid Environments 119: 27-30.
Abstract: The growing period of the deep-rooted, winter-deciduous Capparis spinosa (caper) coincides with the dry season, in the Mediterranean Basin. Roots of wild shrubs of C. spinosa penetrated cracks of a pumice substrate and lengthened in the subterranean environment of the ancient catacombs of Milos Island (Greece); therein, they reached depths of up to 20 m and exploited water present in deep soil layers. Elevated water potential (Ψ) was measured in root segments (Ψr = -0.35±0.06 MPa), a few centimetres from the root apices of C. spinosa found in the catacombs during the dry season. Also, estimates of foliar predawn water potential and gas exchange components, as well as of nocturnal floral water potential of C. spinosa grown on slopes above the subterranean monument were high and reflected the efficiency ofroots in retaining acquisition of water from deep soil layers, during the dry season.
S. Rhizopoulou and H. Pantazi (2015) Constraints on floral water status of successively blossoming Mediterranean plants under natural conditions. Acta Botanica Gallica 162 (1): 97–102
Abstract: The water relations of showy laminar floral tissues (petals and tepals) were studied in twenty Mediterranean plants successively blossoming under ambient conditions. The water and the osmotic potential of floral tissues decline according to the succession of the median day of flowering of the selected plant species. The highest (less negative) value of floral water potential (–0.32 MPa), among the examined species, was measured in petals of Anemone coronaria in March, and the lowest value (–1.25 MPa) in petals of Coridothymus capitatus in June. Low values of water potential of floral tissues coincided with constraints in declining values of osmotic potential, at the onset of the dry period in the Mediterranean region; this apparently resulted in a reduction of turgor of floral tissues of Mediterranean plants subjected to water shortage. The reduced osmotic potential was correlated with enhanced soluble sugar content of floral tissues, presumably contributing to the expansion and water status of flowers under water scarcity, by decreasing water requirements.
Rhizopoulou S., Spanakis E, Argiropoulos A (2015) Study of petal topography of Lysimachia arvensis grown under natural conditions. Acta Botanica Gallica 162(4): 355-364.
Abstract: The present study revealed that adaxial and abaxial petal epidermises of the blue-flowered Lysimachia arvensis consist of elongated, multi-micro-papillate cells, which may aid the rapid petal expansion. The epidermal cells are covered by a wrinkled relief, which is further ornamented by submicron features that increase in size the surface area of lobes; this may be a well-adapted mechanism of the small-sized flowers of L. arvensis with the short life span. The sculpturally increased surface area of adaxial epidermal cells of petals is expected to contribute to optical and adhesive properties, and wettability of the floral tissues. The adaxial and the abaxial petal surfaces of L. arvensis possess submicron cuticular folds, smaller than the sub-wavelength visible spectrum, which reflect radiation of shorter rather than longer wavelengths, whereas intense absorption was detected in the red spectral region. Also, three-celled capitate trichomes with a pigmented spherical head, which are densely distributed at the corolla margins of L. arvensis, may be involved in adhesive, defensive and functional properties of the floral tissues.

Sá-Pessoa J, Amillis S, Casal M, Diallinas G. (2015) Expression and specificity profile of the major acetate transporter AcpA in Aspergillus nidulans. Fungal Genet Biol. 2015 Feb 21. pii: S1087-1845(15)00033-X. doi: 10.1016/j.fgb.2015.02.010.

Abstract: AcpA has been previously characterized as a high-affinity transporter essential for the uptake and use of acetate as sole carbon source in Aspergillus nidulans. Here, we follow the expression profile of AcpA and define its substrate specificity. AcpA-mediated acetate transport is detected from the onset of conidiospore germination, peaks at the time of germ tube emergence, and drops to low basal levels in germlings and young mycelia, where a second acetate transporter is also becoming apparent. AcpA activity also responds to acetate presence in the growth medium, but is not subject to either carbon or nitrogen catabolite repression. Short-chain monocarboxylates (benzoate, formate, butyrate and propionate) inhibit AcpA-mediated acetate transport with apparent inhibition constants (Ki) of 16.89±2.12, 9.25±1.01, 12.06±3.29 and 1.44±0.13mM, respectively. AcpA is also shown not to be directly involved in ammonia export, as proposed for its Saccharomycescerevisiae homologue Ady2p. In the second part of this work, we search for the unknown acetate transporter expressed in mycelia, and for other transporters that might contribute to acetate uptake. In silico analysis, genetic construction of relevant null mutants, and uptake assays, reveal that the closest AcpA homologue (AN1839), named AcpB, is the 'missing' secondary acetate transporter in mycelia. We also identify two major short-chain carboxylate (lactate, succinate, pyruvate and malate) transporters, named JenA (AN6095) and JenB (AN6703), which however are not involved in acetate uptake. This work establishes a framework for further exploiting acetate and carboxylate transport in filamentous ascomycetes.
Argyro Zenetos, Margarita Arianoutsou, Ioannis Bazos, et al. 2015. ELNAIS: A collaborative network on Aquatic Alien Species in Hellas (Greece). Management of Biological Invasions 6(2): 185–196
Abstract: ELNAIS is a dynamic online information platform aiming to collect and report spatial information on Aquatic Alien Species in Greek waters. It covers freshwater, marine and estuarine waters, including not only established aliens but also casual records and cryptogenic species. The ELNAIS system includes: News, List of Greek experts, Literature of findings in Greece, List of species with information on their first introduction date and source as well as photos and distribution maps. Data providers are the scientific community (publications, grey literature, and databases) as well as citizen scientists. ELNAIS provides a useful tool towards national obligations and commitments under both the European and global frameworks in respect to Non Indigenous Species (CBD, WFD, MSFD).